GE17-0406-01
HiTrap® Heparin High Performance
Cytiva 17-0406-01, pack of 5 × 1 mL
Sinônimo(s):
Heparin Affinity Column
About This Item
Produtos recomendados
ligand
porcine heparin
embalagem
pack of 5 × 1 mL
fabricante/nome comercial
Cytiva 17-0406-01
Parâmetros
<4 mL/min flow rate
42 psi
tamanho do leito
7 mm × 25 mm
volume do leito
1 mL
D.I. da coluna
7 mm
tamanho
Tamanho médio da partícula
34 μm
cleaning
5-10
Faixa de trabalho
5-10
Categorias relacionadas
Descrição geral
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Características e benefícios
- Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
- Simple operation with a syringe, a pump or a chromatography system such as AKTA design
- Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
Armazenamento e estabilidade
Nota de análise
Outras notas
Informações legais
produto relacionado
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
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