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Documentos Principais

E6909

Sigma-Aldrich

Gel de MEC

growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

Sinônimo(s):

GFR Basement Membrane Extract (BME)

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

linha de produto

BioReagent

Formulário

liquid

concentração

7-9 mg/mL

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Aplicação

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been used to coat tissue culture plates for different glioma subtypes and C2C12 muscle cells. It has also been used as a scaffold to increase myotube attachment, maturation and survival.
ECM gel can be used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Ações bioquímicas/fisiológicas

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV

Componentes

ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
The growth factor reduction modification allows for more control of medium, better interpretation of results and increased polymerization. The medium also lacks phenol red, which can interfere with some analyses.

Atenção

For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Nota de preparo

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may contain precipitates which do not influence its activity. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Lampson M Fan et al.
The Journal of clinical investigation, 129(8), 3374-3386 (2019-07-23)
Oxidative stress plays an important role in aging-related neurodegeneration. This study used littermates of WT and Nox2-knockout (Nox2KO) mice plus endothelial cell-specific human Nox2 overexpression-transgenic (HuNox2Tg) mice to investigate Nox2-derived ROS in brain aging. Compared with young WT mice (3-4
Gesa Volkers et al.
ACS chemical biology, 13(8), 2320-2328 (2018-07-18)
Polysialyltransferases synthesize polysialic acid on cell surface-expressed glycoconjugates, which is crucial for developing processes and signaling pathways in eukaryotes. Recent advances in cancer research have rendered polysialyltransferases important drug targets because polysialic acid contributes to cancer cell progression, metastasis, and
Muscle Cell and Tissue: Current Status of Research Field (2018)
Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas
Niittykoski M, et al.
Translational Oncology, 10(5), 772-779 (2017)
Ketao Wang et al.
Theranostics, 11(10), 4770-4789 (2021-03-24)
Rationale: The spine is one of the most common metastatic sites of non-small cell lung cancer (NSCLC), and NSCLC spinal metastasis results in serious consequences. Metastatic extravasation of disseminated cancer cells including increased invasiveness, adhesion and transendothelial migration is crucial

Artigos

Cell culture protocols using Sigma ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

Highlighting existing and novel fabrication methods for both, solid and hydrogel-based scaffold for tissue engineering applications.

Attachment Factors for 3-Dimensional Cell Culture

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