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E6654

Millipore

EZview™ Red Anti-c-Myc Affinity Gel

Sinônimo(s):

Anti-c-Myc affinity resin, Anti-c-Myc agarose beads

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.56

prazo de validade

1 yr at 2‑8 °C

classe(s) química(is) do analito

proteins (c-Myc)

técnica(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacidade

~0.05 mg/mL binding capacity

temperatura de armazenamento

2-8°C

Descrição geral

EZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.

Aplicação

EZview® Red Anti-c-Myc Affinity Gel is suitable for:
  • SUMOylation assay
  • Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Características e benefícios

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Outras notas

The affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein.

forma física

1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservative

Informações legais

EZview is a trademark of Sigma-Aldrich Co. LLC
SabreLite is a registered trademark of Pelican Products, Inc.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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