DNAQF
DNA Quantitation Kit, Fluorescence Assay
Quantitation of DNA using bisBenzimide
Sinônimo(s):
dsDNA quantification
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About This Item
Código UNSPSC:
41105500
NACRES:
NA.84
Produtos recomendados
Nível de qualidade
uso
sufficient for 750 assays (2 ml each)
técnica(s)
nucleic acid detection: suitable
temperatura de armazenamento
−20°C
Descrição geral
The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.
Aplicação
DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
- in neonatal rat ventricular myocytes (NRVMs)
- in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
- in mouse embryonic fibroblast cell line (NIH3T3 cells)
Características e benefícios
- Specific for the quantitation of nanogram amounts of DNA
- Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins
Somente componentes do kit
Nº do produto
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- Detailed technical bulletin 1 ea
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10 - Combustible liquids
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