D218
Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse
clone 1A, buffered aqueous solution
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About This Item
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conjugado
unconjugated
Nível de qualidade
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
1A, monoclonal
forma
buffered aqueous solution
peso molecular
antigen ~200 kDa
reatividade de espécies
human (weakly), guinea pig, mouse, rat, rabbit
técnica(s)
immunohistochemistry (frozen sections): 1:200
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1:500
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CACNA1S(779)
mouse ... Cacna1s(12292)
rat ... Cacna1s(682930)
Descrição geral
DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.
Especificidade
Reacts specifically with the α1 subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.
Imunogênio
rabbit muscle transverse tubule dihydropyridine (DHP) receptors.
Aplicação
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.
Ações bioquímicas/fisiológicas
The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca2+ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.
forma física
Solution in phosphate buffered saline containing 0.05% sodium azide.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Philippe Magown et al.
Nature communications, 6, 8506-8506 (2015-10-16)
Neural prostheses can restore meaningful function to paralysed muscles by electrically stimulating innervating motor axons, but fail when muscles are completely denervated, as seen in amyotrophic lateral sclerosis, or after a peripheral nerve or spinal cord injury. Here we show
M E Morton et al.
The Journal of biological chemistry, 262(25), 11904-11907 (1987-09-05)
A monoclonal antibody, mAb 1A, that immunoprecipitates the [3H]PN200-110-binding complex from rabbit skeletal muscle has been used to study the subunit structure of the dihydropyridine-sensitive, voltage-activated calcium channel. Digitonin-solubilized [3H]PN200-110-binding component, purified by wheat germ agglutinin chromatography, sediments as a
F Galbiati et al.
The Journal of biological chemistry, 276(24), 21425-21433 (2001-03-22)
Caveolin-3, a muscle-specific caveolin-related protein, is the principal structural protein of caveolae membrane domains in striated muscle cells. Recently, we identified a novel autosomal dominant form of limb-girdle muscular dystrophy (LGMD-1C) in humans that is due to mutations within the
Bo Van et al.
FEBS letters, 589(10), 1095-1104 (2015-04-07)
Mitsugumin 56 (MG56), also known as the membrane-bound O-acyl-transferase family member hedgehog acyltransferase-like, was identified as a new sarcoplasmic reticulum component in striated muscle. Mg56-knockout mice grew normally for a week after birth, but shortly thereafter exhibited a suckling defect
Christopher R Pierson et al.
Human molecular genetics, 21(4), 811-825 (2011-11-10)
X-linked myotubular myopathy (MTM) is a severe neuromuscular disease of infancy caused by mutations of MTM1, which encodes the phosphoinositide lipid phosphatase, myotubularin. The Mtm1 knockout (KO) mouse has a severe phenotype and its short lifespan (8 weeks) makes it
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