CRISPR21

Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)

• Código UNSPSC: 41106610
• NACRES: NA.51

Propriedades

• forma: liquid
• Nível de qualidade: 200
• embalagem: vial of 50 μL
• concentração: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• aplicação(ões): CRISPR
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

The Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector) is a critical positive control to monitor transfection efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control is a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and hPGK-driven Puromycin and BFP expression. This control requires Cas9 be delivered by a separate vector.

Aplicação

Functional Genomics/Screening/Target Validation

Outras notas

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

Informações legais

CRISPR Use License Agreement

Lentiviral License Agreement

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable