Pular para o conteúdo
Merck
Todas as fotos(5)

Documentos Principais

CMC0022

Sigma-Aldrich

OverExpress™ C43(DE3) Electrocompetent Cells

for the highest protein expression

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(5)

About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

fonte biológica

Escherichia coli

grau

for molecular biology

modo de crescimento

adherent or suspension

morfologia

rod shaped

técnica(s)

microbiological culture: suitable

transformação celular

competent cell type: electrocompetent
transformation efficiency: ≥1 x 109 cfu/μg

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Descrição geral

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Características e benefícios

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Componentes

  • OverExpress C43(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

Informações legais

OverExpress is a trademark of Imaxio, S.A.

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

A Rangunwala et al.
Epidemiology and infection, 142(10), 2147-2154 (2013-12-18)
Crimean-Congo haemorrhagic fever virus (CCHFV) has the propensity to cause nosocomial infections with a high fatality rate. Handling the virus requires biosafety level-4 facilities, limiting accessibility for many laboratories. Advances in molecular techniques have allowed preparation of safe recombinant antigens
Arthur Laganowsky et al.
Nature, 510(7503), 172-175 (2014-06-06)
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments and that lipids can bind to specific sites, for example, in potassium channels. Fundamental questions remain however regarding the extent of

Protocolos

OverExpress™ Electrocompetent Cells

OverExpress™ Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one transformation reaction.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica