LB199

98062321, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propriedades

• product name: LB199, 98062321
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human lymphocyte, trichothiodystrophy, EBV-transformed

Descrição de linhagem celular

LB199 was derived from mononuclear cells from a peripheral blood sample from a 16 year old male. The patient was diagnosed with trichothiodystrophy and was shown to have a severe deficiency in excision repair of UV-irradiation damage. The defect was able to complement all known Xeroderma pigmentosum complementation groups and was not intragenic. This cell line is therefore a representative of a new excision-repair complementation group. The cell line was established by subsequent EBV transformation. Fibroblasts from the same individual have been designated TTD1BR. EBV-transformed lymphoblastoid cells from the mother are available as LB198 (Sigma Catalogue number. 98062320).

Aplicação

DNA repair deficiency studies

Meio de cultura

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rotina de subcultura

Maintain cultures between 3-9 x 100,000 cells/ml; dilute every 1 -2 days to 3 x 100,000 cells/ml; 5% CO₂; 37°C. Cells form floating aggregates.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.