AFRC 58D6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Horse x Mouse/Horse Hybridoma
• embalagem: tube of 5 μg 94120827-DNA-5UG; pkg of vial of cells 94120827-1VL
• ImmunoDon: Horse sub-mandibular lymph node
• Mieloma: AFRC 17D1
• Reatividade: Anti-Haemagglutinin, influenza
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Horse sub-mandibular lymph node x AFRC 17D1

Descrição de linhagem celular

The heterohybridoma AFRC 58D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 58D6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Imunogênio

Influenza A equine 2 virus

Aplicação

Haemagglutinin detection

The isotype of the monoclonal antibody produced by this cell line is horse IgG.

Meio de cultura

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Outras notas

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