G

94101453, rat prostate, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: G, 94101453
• fonte biológica: rat prostate
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Androgen sensitive
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat Dunning R-3327,prostate adenocarcinoma

Descrição de linhagem celular

Derived from a Dunning R-3327 rat with prostatic adenocarcinoma. G is one of a group of variants which developed by a single in vivo passage. It is androgen responsive, moderately fast growing and poorly differentiated. Using this system of rat prostatic adenocarcinomas as a model, it has been demonstrated that prostatic cancer cells can become unstable at any time resulting in the addition of cytogenetically and phenotypically novel clones of cancer cells to the cancer population.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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