Colo 794

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072237, human skin, Not specified

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human skin
• embalagem: tube of 5 μg 94072237-DNA-5UG; pkg of vial of cells 94072237-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human melanoma

Descrição de linhagem celular

Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (Sigma Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.

Aplicação

Cancer studies

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

Meio de cultura

RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; cells can show extensive blebs

Outras notas

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