C6 Cell Line from rat

92090409, rat nerve (glial tumor), Neuronal

• Sinônimo(s): C-6 Cells, RGC-6 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: C6 Cell Line from rat, 92090409
• fonte biológica: rat nerve (glial tumor)
• descrição: Rat glial tumor
• modo de crescimento: Adherent
• cariótipo: 2n = 42
• morfologia: Neuronal
• produtos: S-100 protein, somatotrophin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat glial tumour

Descrição de linhagem celular

Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.

Aplicação

C6 cell line has been used to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions. It has also been used to study the cytotoxic and antioxidant properties of supercritical CO₂ extract (SCE) of old man′s beard (Usnea barbata).

C6 cells are reported to produce elevated levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids

Meio de cultura

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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