70Z/3

91092002, mouse blood, Lymphoblastoid

• Código UNSPSC: 41106514
• fonte biológica: mouse blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Cytoplasmic Immunoglobulin
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: 70Z/3, 91092002
• fonte biológica: mouse blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Cytoplasmic Immunoglobulin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse pre-B lymphocyte

Descrição de linhagem celular

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation