CCRF-CEM

85112105, human blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• product name: CCRF-CEM, 85112105
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: 2n = 46
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian acute lymphoblastic leukaemia

Descrição de linhagem celular

A T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19

Meio de cultura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 2-3x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

Outras notas

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