293-Hektor

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human kidney
• embalagem: tube of 5 μg 05030204-DNA-5UG; pkg of vial of cells 05030204-1VL
• modo de crescimento: Aggregates in suspension
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Embryo Kidney, serum-free

Descrição de linhagem celular

The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the chemically defined protein- and peptide-free Hektor^™ G medium. Cells grow in suspension and tend to form aggregates in static culture. The cells grow as finely dispersed single cell suspension under optimal agitated culture conditions.

Meio de cultura

Hektor^™ G medium + 4mM Glutamine + 5 ug/ml Insulin. The exogeneous insulin supplementation can be reduced or totally eliminated under optimal culture conditions. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO.

Rotina de subcultura

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cell clumps form. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Informações legais

Hektor is a trademark of Cell Culture Technologies