C6870

Anti-COMT antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): Anti-Catechol-O-methyltransferase
• Número MDL: MFCD03454916
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~24 kDa (and ~28 kDa)
• reatividade de espécies: human, mouse, rat
• técnica(s): immunoprecipitation (IP): 5-10 μL using human HepG2 cell lysates; western blot: 1:250-1:500 using whole extracts of mouse and rat liver
• nº de adesão UniProt: P21964
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... COMT(1312) ; mouse ... Comt1(12846) ; rat ... Comt(24267)

Descrição

Descrição geral

Catechol-O-methyltransferase (COMT) is encoded by the gene mapped to human chromosome 22q11.21. It is widely expressed in neuronal and non-neuronal tissues. In mammals, two isoforms of COMT have been identified a cytosolic soluble form (S-COMT) and a membrane-bound form (MB-COMT), whose relative levels differ in various tissues and species.

Especificidade

The antibody recognizes MB-COMT and S-COMT.

Aplicação

Anti-COMT antibody produced in rabbit has been used in enzyme linked immunoassay (ELISA).

Anti-COMT antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μL using human HepG2 cell lysates and western blotting at a dilution of 1:250-1:500 using whole extracts of mouse and rat liver.

Ações bioquímicas/fisiológicas

Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamine neurotransmitters including dopamine, noradrenaline, and adrenaline, their metabolites, and L-DOPA. Its major physiological role is to inactivate biologically active or toxic catechols. Variations in enzymatic activity of COMT result in the pathogenesis of different psychiatric and neurological diseases. COMT inhibitors are important therapeutic agents used in the treatment of Parkinson′s disease.

Catechol-O-methyltransferase (COMT) is referred as HEL-S-98n. The COMT Met/Met genotype individuals can acquire skill in executive function tasks (as multi-UV supervisory control to a higher level and faster than other genotype groups). The genetic variants of COMT alter neurobehavioral functions that are affected by mercury in adults and might modify the adverse neurobehavioral effects of mercury exposure in children.

Descrição-alvo

COMT (Catechol-O-methyltransferase) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)