C3511
Collagen from calf skin
Bornstein and Traub Type I (Sigma Type III), solid
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About This Item
Produtos recomendados
fonte biológica
bovine (calf) skin
forma
solid
técnica(s)
cell culture | mammalian: suitable
solubilidade
aqueous acid: soluble
adequação
suitable for substrate for collagenase
nº de adesão UniProt
temperatura de armazenamento
2-8°C
Informações sobre genes
bovine ... COL1A1(282187)
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Aplicação
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Ações bioquímicas/fisiológicas
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Componentes
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Nota de preparo
Prepared by a modification of the method of Gallop, P.M., and Seifter, S., Meth. Enzymol., VI, 635 (1963).
Outras notas
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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