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Documentos Principais

C-27001

Sigma-Aldrich

Osteoblast Growth Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

Formulário

aqueous solution

embalagem

pkg of 500 ml

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

wet ice

Descrição geral

Osteoblast Growth Medium (Ready-to-use)

Aplicação

Osteoblast Growth Medium has been optimized for the in vitro cultivation of human osteoblasts. The fetal calf serum in the medium is strictly pretested for optimal performance. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial

Qualidade

All lots of PromoCell Osteoblast Growth Medium are subjected to comprehensive quality control tests using primary human osteoblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparo

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / ml

Armazenamento e estabilidade

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rotina de subcultura

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Código de classe de armazenamento

10 - Combustible liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Conteúdo relacionado

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

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