C-23020

Fibroblast Growth Medium 2

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

• Sinônimo(s): Primary Cell Culture Media
• Código UNSPSC: 12352207
• NACRES: NA.71

Propriedades

• Formulário: liquid
• embalagem: pkg of 500 ml
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: wet ice

Descrição

Descrição geral

Fibroblast Growth Medium 2 (Ready-to-use)

Aplicação

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualidade

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparo

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Armazenamento e estabilidade

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rotina de subcultura

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Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable