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Key Documents

C-12071

Sigma-Aldrich

Human Follicle Dermal Papilla Cells (HFDPC)

500,000 cryopreserved cells

Sinônimo(s):

HFDPC cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

fonte biológica

human dermis

embalagem

pkg of 500,000 cells

morfologia

( dermal)

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origem de linhagem celular

Skin

Aplicação

Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region and are provided in a cryopreserved format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.

Qualidade

Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

Click here for more information.

Outras notas

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.

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