A0349

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Número MDL: MFCD03455360
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~200 kDa
• reatividade de espécies: canine, mouse, rat, human
• embalagem: antibody small pack of 25 μL
• validação aprimorada: independent; Learn more about Antibody Enhanced Validation
• técnica(s): immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.; indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.; microarray: suitable; western blot: 1:2,000 using extract of rat brain
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MLLT4(4301) ; mouse ... Mllt4(17356) ; rat ... Mllt4(26955)

Descrição

Descrição geral

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Especificidade

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Imunogênio

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Aplicação

Anti-l-Afadin antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence experiments
• immunocytochemistry
• immunohistochemistry

Ações bioquímicas/fisiológicas

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable