3023
AptaFluor® SAH Methyltransferase Assay
Sinônimo(s):
Methyltransferase Activity Assay
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
41122100
NACRES:
NA.84
Produtos recomendados
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Descrição geral
The AptaFluor® SAH Methyltransferase TR-FRET Assay is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added.
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
View full Transcreener® product list
The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
- Simple, mix-and-read format ideal for HTS.
- Robust MT detection under initial velocity conditions (=20% conversion of SAM to SAH).
- Accommodates SAM concentrations as low as 100 nM and as high as 5 μM.
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering.
View full Transcreener® product list
Quantidade
3023-B = 100 assay, 96-well
3023-1K = 1,000 assay, 384-well
3023-1K = 1,000 assay, 384-well
forma física
Kit with buffered aqueous solutions
Informações legais
AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
Não está vendo a versão correta?
Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
L Latini et al.
Cell death & disease, 5, e1404-e1404 (2014-09-05)
Spinal cord injury (SCI) is a devastating condition of CNS that often results in severe functional impairments for which there are no restorative therapies. As in other CNS injuries, in addition to the effects that are related to the primary
Kalyan C Kondapalli et al.
The Journal of biological chemistry, 287(43), 36239-36250 (2012-09-06)
Human NHA2, a newly discovered cation proton antiporter, is implicated in essential hypertension by gene linkage analysis. We show that NHA2 mediates phloretin-sensitive Na(+)-Li(+) counter-transport (SLC) activity, an established marker for hypertension. In contrast to bacteria and fungi where H(+)
Maria Teresa Viscomi et al.
Autophagy, 8(2), 222-235 (2012-01-18)
Autophagy is the evolutionarily conserved degradation and recycling of cellular constituents. In mammals, autophagy is implicated in the pathogenesis of many neurodegenerative diseases. However, its involvement in acute brain damage is unknown. This study addresses the function of autophagy in
Brian G Coon et al.
The Journal of cell biology, 208(7), 975-986 (2015-03-25)
Endothelial responses to fluid shear stress are essential for vascular development and physiology, and determine the formation of atherosclerotic plaques at regions of disturbed flow. Previous work identified VE-cadherin as an essential component, along with PECAM-1 and VEGFR2, of a
Mirja Tamara Prentzell et al.
Cell, 184(3), 655-674 (2021-01-27)
Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant manner to anchor
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica