137264

HEPES

EMPROVE^® EVOLVE

• Sinônimo(s): Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Fórmula empírica (Notação de Hill): C₈H₁₈N₂O₄S
• Número CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein: 883043
• Número MDL: MFCD00006158
• Código UNSPSC: 12161700

Propriedades

• fonte biológica: synthetic
• Nível de qualidade: 400
• linha de produto: EMPROVE^® EVOLVE
• Formulário: powder
• técnica(s): cell culture | mammalian: suitable
• Impurezas: Endotoxin, microbial, and trace metals; tested
• pH: 4.7-5.3; 6.8-8.2
• faixa de pH útil: 6.8-8.2
• pKa (25 °C): 7.5
• pKa: 7.5
• adequação: suitable for manufacturing use
• atividade externa: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• temperatura de armazenamento: 2-30°C
• cadeia de caracteres SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• chave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Aplicação

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Informações legais

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable