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T4375

Sigma-Aldrich

Tetrazolium Blue Chloride

used in colorimetric determination of reducing compounds

Sinônimo(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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About This Item

Fórmula empírica (Notação de Hill):
C40H32Cl2N8O2
Número CAS:
Peso molecular:
727.64
Beilstein:
3894077
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:

Nível de qualidade

forma

powder

pf

255 °C (dec.) (lit.)

solubilidade

methanol: 50 mg/mL, clear

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

chave InChI

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Aplicação

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Carc. 1B

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Visite a Biblioteca de Documentos

[Development of a novel Francisella tularensis antigen stained with tetrazolium-blue for tularemia microagglutination test].
Celebi B
Mikrobiyoloji bulteni (2013)
Sensitive colorimetric assay of carbidopa and methyldopa using tetrazolium blue chloride in pharmaceutical products.
Issopoulos, P.B.
International Journal of Clinical Pharmacy, 11, 213-217 (1989)
Ternary complex of molybdenum(VI) with 4-nitrocatechol and tetrazolium blue chloride and Its application to extraction-spectrophotometric analysis of ferrous metallurgy products.
Dimitrov., A.N., et al.
Journal of Analytical Chemistry, 62, 122-125 (2007)
J J Li et al.
Neuroscience, 167(3), 665-677 (2010-03-09)
We reported previously that amoeboid microglial cells (AMC) in the developing brain exhibited endothelins (ETs) expression which diminished with advancing age and was undetected in microglia in the more mature brain. This study sought to explore if microglia in the
Preparation of a new tetrazolium salt which yields a blue pigment on reduction and its use in the demonstration of enzymes in normal and neoplastic tissues.
A M RUTENBURG et al.
Cancer research, 10(2), 113-121 (1950-02-01)

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