PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• Sinônimo(s): 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• Código UNSPSC: 41105319
• NACRES: NA.25

Propriedades

• linha de produto: pHast Pack^™
• Nível de qualidade: 100
• Formulário: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white to off-white
• pH: 8.3
• solubilidade: soluble, clear, colorless to faintly yellow
• traços de cátion: Fe: <10 ppm
• atividade externa: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Aplicação

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Características e benefícios

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1