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11663925910

Roche

Mycoplasma PCR ELISA

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About This Item

Código UNSPSC:
12352200

uso

sufficient for 96 reactions

fabricante/nome comercial

Roche

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Especificidade

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Aplicação

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Características e benefícios

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Embalagem

1 kit containing 14 components.

Sequência

Chain Length Positive control: 520 bp.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Somente componentes do kit

Nº do produto
Descrição
  • Lysis Reagent
  • Neutralization Reagent
  • PCR Mix ready-to-use
  • Denaturation Reagent
  • Hybridization Buffer
  • Biotinylated Capture Probe
  • Washing Buffer 10x concentrated
  • Anti-Digoxigenin-POD antibody
  • Conjugate Dilution Buffer
  • TMB Substrate Solution
  • Stop Reagent
  • Positive Control DNA
  • Streptavidin-coated Microplate
  • Adhesive Plate Cover Foils
Ver tudo (14)

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H315,H317,H319

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

B1155

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