Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

11498045910

Roche

Actin RNA Probe, DIG-labeled

greener alternative

solution, pkg of 2 μg

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
41105324

forma

solution

Nível de qualidade

embalagem

pkg of 2 μg

fabricante/nome comercial

Roche

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoria alternativa mais ecológica

temperatura de armazenamento

−20°C

Descrição geral

This antisense RNA probe (human β-actin) is in vitro transcribed in the presence of digoxigenin (DIG)-UTP. The transcript has a length of 588 bases. 550 bases are complementary to the 5′ region of human β-actin mRNA (nucleotides 69 to 6l8, EMBL: HSAC07). The additional 25 bases at the 5′ end, and the 13 additional bases at the 3′ end of the transcript are specific for the promoter/polylinker region of the transcription vector.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Aplicação

Actin RNA Probe, DIG-labeled, is specifically useful for evaluating the quality and quantity of various RNA species and can be used:
  • in In situ hybridization (for example, as a control in mRNA detection)
  • for quality control in the construction of cDNA libraries
  • in northern blot analysis to evaluate RNA from various human cell lines and tissue samples

Componentes

Actin RNA Probe, DIG-labeled, is supplied in autoclaved, DEPC-treated water.

Sequência

Agarose gel electrophoresis under denaturing conditions and subsequent northern blot analysis reveal a defined band of 588 bases.

Nota de preparo

Working solution: Dilute Sample
  • Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
    The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
  • Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
    Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
    concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

Storage conditions (working solution): During assay, the preparation should be maintained at 0 °C.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 2

1 of 2

E Tsuruga et al.
Journal of dental research, 81(11), 771-775 (2002-10-31)
The elastic system fibers consist of three types--oxytalan, elaunin, and elastic fibers--differing in their relative microfibril and elastin contents. All three types are found in human gingiva, but human periodontal ligaments contain only elastin-free fibers. We examined cultured human gingival

Artigos

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica