11427857910
Roche
Fluorescein-12-UTP
≥85% (HPLC), solution
Sinônimo(s):
Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
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About This Item
Produtos recomendados
Nível de qualidade
Ensaio
≥85% (HPLC)
Formulário
solution
peso molecular
1034.4
embalagem
pkg of 25 μL (250 nmol; 10 mM)
fabricante/nome comercial
Roche
temperatura de armazenamento
−20°C
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Descrição geral
Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.
Aplicação
Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Outras notas
For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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