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Merck
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Documentos Principais

10652202001

Roche

Transferrin

from human serum

Sinônimo(s):

serum glycoprotein, transferrin

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About This Item

Código UNSPSC:
12352207

Nível de qualidade

esterilidade

0.2 μm filtered

Formulário

solution

embalagem

pkg of 20 mL (30 mg/ml)

fabricante/nome comercial

Roche

técnica(s)

cell culture | mammalian: suitable

cor

clear red

solubilidade

water: miscible

nº de adesão UniProt

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... TF(7018)

Descrição geral

Transferrin is a serum glycoprotein that participates in the transport of iron from absorption and storage sites to tissue cells. The iron-bound transferrin, also referred to as ferrotransferrin, binds to specific cell surface receptors on growing cells and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded.

Aplicação

Transferrin, similar to insulin, is a component of most serum-free media formulations. The main function of transferrin is to transport iron into cells by receptor-mediated endocytosis.
Transferrin from human serum has been used as a supplement in:
  • differentiation medium
  • keratinocyte medium
  • haemogenic endothelium culture medium
  • embryoid-body medium
  • blast colony forming cell-culture medium

forma física

Solution, 30 mg/ml in IMDM, filtered through 0.2 μm pore size membrane. Iron saturation is below 1%. This product is Apo-Transferrin.

Nota de preparo

Working concentration: 1 to 100 μg/ml

Armazenamento e estabilidade

Store at 2 to 8 °C. (lyophilizate and solution)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


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Journal of visualized experiments : JoVE, (49)(49), doi:10-doi:10 (2011-04-15)
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Quantification of Mouse Hematopoietic Progenitors? Formation Using Time-lapse Microscopy and Image Analysis
Bergiers I, et al.
Bio-protocol, 9(1), e3137-e3137 (2019)
Iron deficiency affects early stages of embryonic hematopoiesis but not the
endothelial to hematopoietic transition.
Shvartsman M, et al.
bioRxiv (2018)
Özge Vargel et al.
Scientific reports, 6, 21518-21518 (2016-02-20)
The endothelial to haematopoietic transition (EHT) is a key developmental process where a drastic change of endothelial cell morphology leads to the formation of blood stem and progenitor cells during embryogenesis. As TGFβ signalling triggers a similar event during embryonic
T J M Welting et al.
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