10109339001

Sorbitol Dehydrogenase (SDH)

from sheep liver

• Sinônimo(s): SDH, Sorbitol Dehydrogenase, sorbitol
• Número da licença da enzima: 1.1.1.14 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: sheep liver
• Formulário: lyophilized
• atividade específica: ~40 units/mg protein (At 25 °C with D-fructose as the substrate.)
• peso molecular: 115 kDa
• embalagem: pkg of 10 mg (60 mg lyophilizate)
• fabricante/nome comercial: Roche
• concentração: 0.16 mg/mL Biuret
• técnica(s): activity assay: suitable
• cor: white
• pH ideal: 7.5
• solubilidade: water: 60 mg/mL, clear, colorless
• adequação: suitable for ELISA applications
• nº de adesão NCBI: XP_004010700
• nº de adesão UniProt: P07846
• aplicação(ões): life science and biopharma
• atividade externa: ADH <0.00182%; GIDH <0.00152%; Glucose-DH <0.00182% (NAD as coenz.); LDH <0.00167%; MDH <0.00198%; NADH oxidase 0.00532%
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: sheep ... SORD(101115280)

Descrição

Descrição geral

L-iditol:NAD⁺ 5′-oxidoreductase
Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis.

Aplicação

Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses).

Ações bioquímicas/fisiológicas

Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of D-sorbitol to D-fructose and forms a part of the sorbitol pathway, which is a crucial bypass to glycolysis for glucose metabolism. This pathway has been found to be associated with the accumulation of sorbitol in lens, which results in diabetic cataractogenesis. SDH also oxidizes various polyols and other secondary alcohols into their corresponding ketones.

Qualidade

Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each.

Definição da unidade

One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed.

forma física

Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose).

Nota de preparo

Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer.
Stabilizers: Maltose is used as stabilizer.
Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks.

Armazenamento e estabilidade

Store at 2 to 8 °C. (Store the lyophilizate dry.)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash