SCR011
B6-White Murine ES Cell Line
The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function.
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About This Item
Código UNSPSC:
41106509
eCl@ss:
32011203
NACRES:
NA.81
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
fabricante/nome comercial
B6-White
técnica(s)
cell culture | stem cell: suitable
entrada
sample type: mouse embryonic stem cell(s)
Condições de expedição
liquid nitrogen
Descrição geral
The generation of gene-modified mice, created by homologous recombination in embryonic stem (ES) cells, has become a fundamental tool for analyzing gene function. The influence of genetic background on phenotype has been shown to be an important consideration in selection of a mouse model. Furthermore, the time required to achieve congenic status, which can be 18-24 months has further stimulated demand for targeting in pure inbred strains including C57BL/6 (B6) mice.
Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.
For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).
STRAIN:C57BL/6-tyr(c-2J) albino mice
GENDER: Male
KARYOTYPE: 40XY
Millipore′s B6-White Murine ES cell line is the first commercially available C57BL/6 tyrc-2J albino line that allows for rapid coat-color determination of successful chimerism in the C57BL/6 mouse strain. When B6-White ES cells are injected into C57BL/6 blastocysts, chimeric mice are easily identified by their coat color (a mix of black and white patches), whilst non-chimeric littermates are black. These cells allow for the efficient generation of gene-targeted mice in a pure C57BL/6 genetic background, thus providing more experimental flexibility. Additionally the use of these cells can lower production costs by eliminating the need to maintain an albino blast donor colony, in order to assess chimerism by coat color when targeting in the C57BL/6 strain.
For germline transmission confirmation by coat color, it is recommended that male chimeras are mated with homozygous females of the B6-white strain (strain name: C57BL/6J-Tyrc-2J/J - available from Jackson Labs, stock number 000058).
STRAIN:C57BL/6-tyr(c-2J) albino mice
GENDER: Male
KARYOTYPE: 40XY
Descrição de linhagem celular
Embryonic Stem Cells
Aplicação
Research Category
Stem Cell Research
Stem Cell Research
forma física
Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO
Armazenamento e estabilidade
Place vials in the vapor phase of liquid nitrogen storage immediately upon receipt until it is convenient to proceed to subculture.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(7), 3583-3596 (2018-02-18)
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