SCC162
CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line
Human
Sinônimo(s):
CFTE29o, CFTE-29o-
About This Item
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product name
CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line, CFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways.
fonte biológica
human
técnica(s)
cell based assay: suitable
cell culture | mammalian: suitable
Condições de expedição
ambient
Categorias relacionadas
Descrição geral
CFTE29o- is a human CF tracheal epithelial cell line, derived from a 21-yr old male CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . The cell line express keratin, indicating an epithelial cell origin, along with a calcium-dependent cell adhesion protein, cellCAM 120/80 , and ZO-1 indicating the ability to form tight junctions. CFTE29o- displays ion transport properties characteristic of cystic fibrosis such as defective cAMP-dependent chloride transport. Upon treatment with the calcium ionophore, ionomycin, CFTE29o- secretes chloride, albeit at reduced levels relative to normal cells. The cells express CFTR mRNA, and small amounts of CFTR protein as assessed by Western blot hybridization . Karyotype analysis indicates that 70% of the cells contain two copies of chromosome 7 .
CFTE29o- is a useful model for cystic fibrosis and for studying chloride ion transport and ion transport in human airways. The cells are useful for testing candidate chemotherapeutic drugs for their potential as treatments for ameliorating CF pathologies.
Descrição de linhagem celular
Aplicação
Qualidade
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Armazenamento e estabilidade
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.
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