OP97
Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)
liquid, clone 22C2, Calbiochem®
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About This Item
Código UNSPSC:
12352203
clone:
22C2, monoclonal
application:
reatividade de espécies:
human
citations:
4
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
22C2, monoclonal
Formulário
liquid
contém
≤0.1% sodium azide as preservative
reatividade de espécies
human
fabricante/nome comercial
Calbiochem®
condição de armazenamento
do not freeze
Isotipo
IgG1
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... MTOR(2475)
Descrição geral
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SpAG8 mouse myeloma cells. Recognizes the ~290 kDa mTOR/FRAP protein.
Recognizes the ~290 kDa mTOR protein in HEK293 and Jurkat cells.
This Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) is validated for use in Immunoblotting, Immunoprecipitation for the detection of mTOR/FRAP (Ab-2).
Imunogênio
Human
a synthetic peptide corresponding to amino acids 230-240 of human TOR
Aplicação
Immunoblotting (1.7 g/ml, see application references)
Immunoprecipitation (1-10 g/ml, see application references)
Embalagem
Please refer to vial label for lot-specific concentration.
Advertência
Toxicity: Standard Handling (A)
forma física
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Nota de análise
Positive Control
Jurkat or HeLa cells
Jurkat or HeLa cells
Outras notas
Kimura N et al. 2003. Genes Cells8, 65.
Sekulic A et al. 2000. Cancer Res.60, 3504.
Hosoi, H. 1999. Cancer Res.59, 886.
Alarcon, C.M., et al. 1996. Genes Dev.10, 279.
Freeman, K., and Livi, G.P. 1996. Gene172, 143.
Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
Cardenas, M.E., and Heitman, J. 1995. EMBO J.14, 5892.
Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem.270, 27531.
Sabatini, D.M., et al. 1995. J. Biol. Chem.270, 20875.
Zheng, X.F., et al. 1995. Cell82, 121.
Brown, E.J., et al. 1994. Nature369, 756.
Sabatini, D.M., et al. 1994. Cell78, 35.
Stan, R., et al. 1994. J. Biol. Chem.269, 32027.
Kunz, J., et al. 1993. Cell73, 585.
Heitman, J., et al. 1991. Science253, 905.
Sekulic A et al. 2000. Cancer Res.60, 3504.
Hosoi, H. 1999. Cancer Res.59, 886.
Alarcon, C.M., et al. 1996. Genes Dev.10, 279.
Freeman, K., and Livi, G.P. 1996. Gene172, 143.
Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
Cardenas, M.E., and Heitman, J. 1995. EMBO J.14, 5892.
Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem.270, 27531.
Sabatini, D.M., et al. 1995. J. Biol. Chem.270, 20875.
Zheng, X.F., et al. 1995. Cell82, 121.
Brown, E.J., et al. 1994. Nature369, 756.
Sabatini, D.M., et al. 1994. Cell78, 35.
Stan, R., et al. 1994. J. Biol. Chem.269, 32027.
Kunz, J., et al. 1993. Cell73, 585.
Heitman, J., et al. 1991. Science253, 905.
The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
M C Lorenz et al.
The Journal of biological chemistry, 270(46), 27531-27537 (1995-11-17)
The antifungal, immunosuppressive compound rapamycin arrests the cell cycle in G1 in both yeast cells and T-lymphocytes. Previous genetic studies in yeast identified mutations in three genes, FPR1 (FKBP12), TOR1, and TOR2, which confer rapamycin resistance, and genetic findings implicated
R Stan et al.
The Journal of biological chemistry, 269(51), 32027-32030 (1994-12-23)
The yeast TOR1 and TOR2 proteins were previously discovered as putative targets of the immunosuppressive drug rapamycin. Although their cellular function is unknown, they are predicted to be at least 215 kDa in size and possess a C-terminal phosphatidylinositol (PI)
J Kunz et al.
Cell, 73(3), 585-596 (1993-05-07)
The yeast TOR2 gene encodes an essential 282 kd phosphatidylinositol (PI) 3-kinase homolog. TOR2 is related to the catalytic subunit of bovine PI 3-kinase and to yeast VPS34, a vacuolar sorting protein also shown to have PI 3-kinase activity. The
C M Alarcon et al.
Genes & development, 10(3), 279-288 (1996-02-01)
In complex with the prolyl isomerase FKBP12, the natural product rapamycin blocks signal transduction in organisms as diverse as yeast and man. The yeast targets of FKBP12-rapamycin, TOR1 and TOR2, are large proteins with homology to lipid and protein kinases.
K Freeman et al.
Gene, 172(1), 143-147 (1996-06-12)
The TOR genes were first identified in Saccharomyces cerevisiae by the isolation of mutants which exhibit dominant resistance to the immunosuppressive and antifungal drug rapamycin (Rm). The originally characterized Rm-resistant (RmR) TOR1-1 and TOR2-1 alleles contain an Arg in place
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