NE1015
Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)
liquid, clone SMI-22, Calbiochem®
Sinônimo(s):
Anti-GFAP Cocktail
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
SMI-22, monoclonal
Formulário
liquid
contém
≤0.1% sodium azide as preservative
reatividade de espécies
human, bovine, mouse, guinea pig, porcine, sheep, canine, rat, chicken
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG2b
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... GFAP(2670)
Descrição geral
Mouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
Recognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
Imunogênio
Bovine
purified bovine GFAP protein
Aplicação
ELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
Advertência
Toxicity: Standard Handling (A)
forma física
Undiluted ascites.
Reconstituição
Upon initial thaw, aliquot and freeze (-20°C).
Nota de análise
Positive Control
Astrocytes or cytoskeletal preparations
Astrocytes or cytoskeletal preparations
Outras notas
This cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
Vick, W.W., et al. 1987. Acta. Cytol.31, 816.
McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.
Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.
McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol.45, 692.
Pegram, C.N., et al. 1985. Neurochem. Pathol.3, 119.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
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