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MCVD2MAG-77K

Millipore

MILLIPLEX® Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

reatividade de espécies

mouse

Nível de qualidade

fabricante/nome comercial

Milliplex®

assay range

accuracy: 84-106%
standard curve range: 137-100,000 pg/mL
(CD40L)

standard curve range: 14-10,000 pg/mL
(Endocan-1)

standard curve range: 274-200,000 pg/mL
(Follistatin)

standard curve range: 34-25,000 pg/mL
(CXCL16, Oncostatin M)

standard curve range: 4-3,000 pg/mL
(PIGF-2)

standard curve range: 686-500,000 pg/mL
(LIGHT, Troponin I)

standard curve range: 69-50,000 pg/mL
(Troponin T)

inter-assay cv: <20%
intra-assay cv: <10%

técnica(s)

multiplexing: suitable

método de detecção

fluorometric (Luminex xMAP)

Condições de expedição

wet ice

Descrição geral

Cardiovascular disease (CVD), particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk
factors.

MILLIPLEX® Mouse CVD Panel 2 is a 9 plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, or tissue culture samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I and Troponin T. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular
The MILLIPLEX® MAP Mouse Cardiovascular Disease Panel 2 is used for the simultaneous quantification of any of the following analytes in serum, plasma, or culture supernatant samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I, Troponin T.

Especificidade

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Aplicação

  • Analytes: sCD40L, CXCL16, Endocan-1 (ESM-1), Follistatin, LIGHT, Oncostatin M (OSM), , Placental Growth Factor 2 (PIGF-2), Troponin I (TnI), Troponin T (TnT)
  • Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
  • Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture supernatants may require dilution in appropriate control medium
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Cardiovascular Disease
  • Research Subcategory: Metabolism

Características e benefícios

Design your multiplex kit by choosing available analytes within this panel.

Embalagem

Everything you need in a single kit.

Componentes

Mouse CVD Panel 2 Standard, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 1, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 bottle, 4 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse CVD Panel 2 Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Armazenamento e estabilidade

Recommended storage for kit components is 2 - 8°C.

Outras notas

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.

Informações legais

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Skull and crossbonesEnvironment

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificados de análise (COA)

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David Sánchez-Infantes et al.
Obesity (Silver Spring, Md.), 25(1), 85-93 (2016-10-06)
Since oncostatin m (OSM) is elevated in adipose tissue in conditions of obesity and type 2 diabetes in mice and humans, the aim of this study was to determine whether this cytokine plays a crucial role in the impairment of

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