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Key Documents

MAC112

Sigma-Aldrich

Anti-S•Tag fusion protein Antibody

clone 26A4.1.2, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

26A4.1.2, monoclonal

reatividade da espécie (prevista por homologia)

all

técnica(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

The S•Tag fusion protein is one of many polypeptide tags used to facilitate the identification and purification of many targets of interest. The gene encoding the S•Tag peptide can be added into a vector with another gene that encodes a target of interest. Such vectors, which are available through the Novagen product line, are subsequently transfected into various mammalian, bacterial, or insect cell cultures. Because the target of interest and the S•Tag peptide are expressed as one protein, identification of the target is facilitated with the use of antibodies that are specific for the respective fusion tag. Optional purification of the target may be performed using chromatography techniques that allow for the binding and removal of the respective fusion tag, if desired.

Imunogênio

Linear peptide corresponding to S•Tag fusion proteins.

Aplicação

Detect S•Tag fusion protein using this Anti-S•Tag fusion protein Antibody validated for use in Western Blotting, ICC, Immunoprecipitation.
Immunocytochemistry Analysis: A representative lot detected S•Tag fusion proteins in transfected COS-7 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitate S•Tag fusion proteins in IP.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualidade

Evaluated by Western Blot in Posi-Tag Epitope Tag Control.

Western Blot Analysis: 0.05 µg/mL of this antibody detected S•Tag fusion proteins in 5 µL of Posi-Tag Epitope Tag Control.

Descrição-alvo

~45 kDa observed. The molecular weight of S•Tag fusion protein is not 45 kDa The Posi-Tag Epitope Tag Control, which contains an S•Tag fusion protein, is observed at ~45 kDa

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Posi-Tag Epitope Tag Control

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Bandarigoda Nipunika Somatilaka et al.
Developmental cell, 54(6), 710-726 (2020-07-24)
The mechanisms underlying subcellular targeting of cAMP-generating adenylyl cyclases and processes regulated by their compartmentalization are poorly understood. Here, we identify Ankmy2 as a repressor of the Hedgehog pathway via adenylyl cyclase targeting. Ankmy2 binds to multiple adenylyl cyclases, determining

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