MABT397

Anti-CEACAM1/CD66a Antibody, clone B3-17

clone B3-17, from mouse

• Sinônimo(s): Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: B3-17, monoclonal
• reatividade de espécies: human
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_001020083
• nº de adesão UniProt: P13688
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CEACAM1(634)

Descrição

Descrição geral

CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is thought to be a member of the immunoglobulin superfamily and is known as an epithelial tumor suppressor and an angiogenic growth factor. It has also been linked to the actin-based cytoskeleton. CEACAM1 is also known as a cellular receptor for a number of human mucosa pathogenic bacteria. The loss of activity of CEACAM1 has been related to the development of colorectal cancer.

Imunogênio

Recombinant human CEACAM1/CD66a.

Aplicação

ELISA Analysis: 10 µg/mL from a representative lot detected human CEACAM1/CD66a by ELISA (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Flow Cytometry Analysis: 10 µg/mL from a representative lot detected the exogenously expressed human CEACAM1/CD66a on the surface of transfected HeLa cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a on the surface of HT-29 human colorectal carcinoma cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a immunoreactivity in human jejunum tissue (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Western Blotting Analysis: 10 µg/mL from a representative lot detected the exogensously expressed human CEACAM1/CD66a extracellular domain-Fc fusion in lysates from transfected cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Flow Cytometry Analysis: Representative lots, either unconjugated or FITC-conjugated, detected CECAM1 immunoreactivity on the surface of human peripheral blood naïve and memory B-cells (Khairnar, V., et al. (2015). Nat. Commun. 6:6217; Seifert, M., et al. (2015). Proc. Natl. Acad. Sci. 112(6):E546-555).

Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a induction on the surface of normal human bronchial epithelial (NHBE) cells stimulated with poly(I:C) or interferons (Klaile, E., et al. (2013) Respir Res. 14:85).

Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a expression on the surface of 2 day-starved human epithelial (HT29, T102/3) and endothelial (AS-M.5) cells, as well as multivesicular bodies (MVBs) derived from these cells (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).

Immunocytochemistry Analysis: A representative lot detected CEACAM1/CD66a immunoreactivity on the surface of normal human bronchial epithelial (NHBE) cells by indirect immunofluorescence staining (Klaile, E., et al. (2013) Respir Res. 14:85).

Immunohistochemistry Analysis: A representative lot detected CEACAM1/CD66a in paraffin-embedded human lung cancer tissue sections (Klaile, E., et al. (2013) Respir Res. 14:85).

Western Blot Analysis: A representative lot detected CEACAM1/CD66a in 2 day-starved AS-M.5 human endothelialn cells and AS-M.5-derived multivesicular bodies (MVBs) (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).

This Anti-CEACAM1/CD66a Antibody, clone B3-17 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of human CEACAM1/CD66a.

Qualidade

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CEACAM1/CD66a in 10 µg of HepG2 cell lysate.

Descrição-alvo

~160 kDa observed. Target band size appears larger than the calculated molecular weights of 57.56/53,80 kDa (pro-/mature isoform 1; BGPa, CEACAM1-4L, TM1-CEA), 45.95/42.19 kDa (pro-/mature isoform 2; BGPg, CEACAM1-4C1), 35.30/31.54 kDa (pro-/mature isoform 3; BGPh, CEACAM1-3), 38.58/34.81 kDa (pro-/mature isoform 4; BGPi, CEACAM1-3C2), 50.35/46.58 kDa (pro-/mature isoform 5; BGPy, CEACAM1-3AL), 46.91/43.15 kDa (pro-/mature isoform 6; BGPb, CEACAM1-3L, TM2-CEA), 27.43/23.67 kDa (pro-/mature isoform 7; BGPx, CEACAM1-1L), 50.52/46.76 kDa (pro-/mature isoform 8; BGPc, CEACAM1-4S, TM3-CEA), 43.06/39.30 kDa (pro-/mature isoform 9; BGPz, CEACAM1-3AS), 51.15/47.38 kDa (pro-/mature isoform 10), 39.87/36.11 kDa ((pro-/mature isoform 11; BGPd, CEACAM1-3S) due to glycosylation. Uncharacterized bands may be observed in some lysates.

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable