MABS2039

Anti-SUMO-2/3 Antibody, clone 8A2

clone 8A2, from mouse

• Sinônimo(s): Small ubiquitin-related modifier 2, HSMT3, SMT3 homolog 2, SUMO-3, Zentrin-2, Ubiquitin-like protein SMT3B, Smt3B
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 8A2, monoclonal
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μg
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_008867.2; NP_008868.3
• nº de adesão UniProt: P55854; P61956
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SUMO2(6613)

Descrição

Descrição geral

Small ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off.

Especificidade

Clone 8A2 detects SUMO-2/3 in human cells.

Imunogênio

GST-tagged full length recombinant human SUMO-2 protein.

Aplicação

Anti-SUMO-2/3, clone 8A2, Cat. No. MABS2039, is a mouse monoclonal antibody that detects SUMO-2 and SUMO-3 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected SUMO-2/3 in Western Blotting applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).

Immunoprecipitation Analysis: A representative lot detected SUMO-2/3 in Immunoprecipitation applications (Zhu, S., et. al. (2009). Mol Cell. 33(5):570-80).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cell lysate (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells (Courtesy of Christine Lee, Matunis Lab, John Hopkins University, Baltimore, Maryland USA).

Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 in Immunocytochemistry applications (Zhang, X.D., et. al. (2008). Mol Cell. 29(6):729-41; Rao, H.B., et. al. (2017) Science. 355(6323):403-407).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SUMO-2/3 in HeLa cells.

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected SUMO-2/3 in HeLa cell lysate.

Descrição-alvo

~19 kDa observed; 10.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash