MABS195
Anti-Pan-Ras Antibody, clone RAS 10
clone RAS 10, from mouse
Sinônimo(s):
GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase Kras, N-terminally processed
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
RAS 10, monoclonal
reatividade de espécies
human
técnica(s)
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG2aκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... KRAS(3845)
Descrição geral
The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
Imunogênio
Recombinant protein corresponding to human Pan-Ras.
Aplicação
Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Qualidade
Evaluated by Western Blot in MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.
Descrição-alvo
~21 kDa observed
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
MCF7 cell lysate
MCF7 cell lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Production and characterization of anti-RAS p21 monoclonal antibodies.
Hamer, P J, et al.
Hybridoma, 9, 573-587 (1990)
Won-Ji Ryu et al.
Experimental & molecular medicine, 52(5), 832-842 (2020-05-28)
Triple-negative breast cancer (TNBC) is a severe and heterogeneous disease that lacks an approved targeted therapy and has a poor clinical outcome to chemotherapy. Although the RAS-ERK signaling axis is rarely mutated in TNBC, ~50% of TNBCs show an increased
Sang-Kyu Lee et al.
EMBO reports, 19(12) (2018-11-11)
RAS proteins play critical roles in various cellular processes, including growth and transformation. RAS proteins are subjected to protein stability regulation via the Wnt/β-catenin pathway, and glycogen synthase kinase 3 beta (GSK3β) is a key player for the phosphorylation-dependent RAS
Won-Ji Ryu et al.
Cancers, 11(4) (2019-04-11)
: Treatment of advanced gastric cancer patients with current standard chemotherapeutic agents frequently results in resistance, leading to poor overall survival. However, there has been no success in developing strategies to overcome it. We showed the expression levels of both
Alisa Damnernsawad et al.
Haematologica, 107(1), 77-85 (2020-12-31)
Drug resistance impedes the long-term effect of targeted therapies in acute myeloid leukemia (AML), necessitating the identification of mechanisms underlying resistance. Approximately 25% of AML patients carry FLT3 mutations and develop post-treatment insensitivity to FLT3 inhibitors, including sorafenib. Using a
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