MABS1904

Anti-RAMP1 Antibody, clone 4F8.1

clone 4F8.1, from mouse

• Sinônimo(s): Receptor activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4F8.1, monoclonal
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μg
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_005846
• nº de adesão UniProt: O60894
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... RAMP1(10267)

Descrição

Descrição geral

Receptor activity-modifying protein 1 (UniProt: O60894; also known as Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, RAMP1) is encoded by the RAMP1 gene (Gene ID: 10267) in human. RAMP1 is a single-pass type I membrane protein that is involved in the transports of calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane and acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. RAMP1 lacks glycosylation sites and does not migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin. It is expressed in many tissues including the uterus, bladder, brain, pancreas, retina, and gastro-intestinal tract. It is synthesized with a signal peptide (aa 1-26), which is cleaved to generate the mature form. Mature RAMP1 has an extracellular domain (aa 27-117); a transmembrane domain (aa 118-138); and a short cytoplasmic tail (aa 139-148). RAMP1 is reported to acts as a vasodilation factor and mice deficient in RAMP1 exhibit inflammatory responses with a transient, but significant, increase in serum calcitonin-gene-related peptide levels and pro-inflammatory cytokines. (Ref.: Nakayama, T., et al. (2017). J. Hum. Hypertens. 31(5); 361-365).

Especificidade

Clone 4F8.1 specifically detects Receptor activity-modifying protein 1 (RAMP1) in human cells. It targets an epitope within 91 amino acids from the internal region.

Imunogênio

GST/His-tagged recombinant fragment corresponding to 91 amino acids from the internal region of human Receptor activity-modifying protein 1 (RAMP1).

Aplicação

Anti-RAMP1, clone 4F8.1, Cat. No. MABS1904, is a mouse monoclonal antibody that detects Receptor activity-modifying protein 1 (RAMP1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RAMP1 in human uterus and human colon tissue sections.

Research Category
Signaling

Qualidade

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAMP1 in human brain tissue lysate.

Descrição-alvo

~17 kDa observed; 16.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable