MABS1310

Anti-Apolipoprotein A-I Antibody, clone Wt20-7

clone Wt20-7, from mouse

• Sinônimo(s): Apo-AI, ApoA-I, Apolipoprotein A1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: Wt20-7, monoclonal
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• técnica(s): ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NP_000030
• nº de adesão UniProt: P02647
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... APOA1(335)

Descrição

Descrição geral

Apolipoprotein A-I (UniProt: P02647; also known as Apo-AI, ApoA-I, Apolipoprotein A1) is encoded by the APOA1 gene (Gene ID: 335) in human. Apo A-1 is a major protein of plasma high density lipoprotein (HDL) and is also found in chylomicrons. It plays a major role in transporting cholesterol from peripheral tissues to the liver. It is synthesized mainly in the liver with a signal peptide (aa 1-18), which is proteolytically cleaved to generate the mature protein. Apo A-1 also serves as a co-factor for lecithin cholesterolacyltransferase (LCAT) that is involved in the formation of cholesteryl esters. Elevated levels of oxidized Apo A-1 (on Methionine 110 and 136) have been linked to increased risk for coronary artery disease. Mutations in APOA1 gene have also been linked to HDL deficiencies that lead to premature coronary artery disease and a reduction in cellular cholesterol efflux. Mutations in APOA1 gene have also shown to result in amyloidosis with extensive visceral amyloid deposit and in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as familial amyloid polyneuropathy type III.

Especificidade

Clone Wt20-7 specifically detects human Apolipoprotein A-I.

Imunogênio

GST-tagged full-length human recombinant Apolipoprotein A-I

Aplicação

Anti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Western Blotting.

ELISA Analysis: A representative lot detected Apolipoprotein A-I in ELISA applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988).

Western Blotting Analysis: A representative lot detected Apolipoprotein A-I in Western Blotting applications (Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Immunocytochemistry Analysis: A representative lot detected Apolipoprotein A-I in Immunocytochemistry applications (Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Dot Blot Analysis: A representative lot detected Apolipoprotein A-I in Dot Blot applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988; Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Research Category
Signaling

Qualidade

Evaluated by Western Blotting in human fetal liver tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apolipoprotein A-I in human fetal liver tissue lysate.

Descrição-alvo

~28 kDa observed; 30.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable