MABN1534

Anti-TREM-2 Antibody, clone 10H4.2

clone 10H4.2, from mouse

• Sinônimo(s): Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 10H4.2, monoclonal
• reatividade de espécies: human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_061838
• nº de adesão UniProt: Q9NZC2
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TREM2(54209)

Descrição

Descrição geral

Triggering receptor expressed on myeloid cells 2 (UniProt Q9NZC2; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the TREM2 gene (Gene ID 54209) in human. TREM2 is an Ig-like V-type receptor expressed by populations of myeloid cells in the central nervous system and periphery. TREM2 is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. Apolipoprotein E (apoE) is reported to function as a high-affinity TREM2 ligand. ApoE binding enhances the phagocytosis of apoptotic N2a cells by primary microglia cells, and greatly reduced ApoE binding is seen with AD-associated TREM2 R47H mutatant. TREM2 is produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the mature single-transmembrane (a.a. 175-195) protein with a large extracellular region (a.a. 19-174) that contains the V-type Ig-like domain (a.a. 29-112) and a cytoplasmic tail (a.a. 196-230). The short cytoplasmic tail contains no signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction. TREM2 gene mutations and polymorphisms are linked to several neurological disorders, including Alzheimer′s disease (AD), frontotemporal dementia (FTD), Parkinson′s disease (PD), and Nasu-Hakola disease (a.k.a. PLOSL or polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy).

Especificidade

Clone 10H4.2 recognizes a cytoplasmic domain epitope that is not present in the spliced isoforms 2&3 reported by UniProt (Q9NZC2).

Imunogênio

Epitope: Cytoplasmic domain.

KLH-conjugated linear peptide corresponding to a cytoplasmic domain sequence of human TREM-2.

Aplicação

Anti-TREM-2 Antibody, clone 10H4.2 is an antibody against TREM-2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TREM-2 in human bone marrow, spleen and cerebellum tissue sections.

Research Category
Neuroscience

Research Sub Category
Neuroinflammation & Pain

Qualidade

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TREM-2 in 10 µg of human spleen tissue lysate.

Descrição-alvo

~30 kDa oberved. Target band size appears larger than the calculated moleuclar weights of 25.45 kDa (pro-form) and 23.45 kDa (mature form) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable