Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

MABF2778

Sigma-Aldrich

Anti-IRF-3 Antibody, clone AR-1

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

AR-1, monoclonal

peso molecular

calculated mol wt 47.22 kDa
observed mol wt ~51 kDa

reatividade de espécies

rhesus macaque, human

embalagem

antibody small pack of 100 μL

técnica(s)

ELISA: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Categorias relacionadas

Descrição geral

Interferon regulatory factor 3 (UniProt: Q14653; also known as IRF-3) is encoded by the IRF3 gene (Gene ID: 3661) in human. IRF-3 is a transcription factor that is constitutively expressed in a variety of tissues and plays a role in intracellular immune response against DNA and RNA viruses. It regulates the transcription of type I interferon genes (IFN-a and IFN-b) and interferon-stimulated genes by binding to an interferon-stimulated response element in their promoters. IRF-3 contains a DNA-binding domain (aa 5-111), nuclear export signal (aa 139-149), IRF-interacting domain, and a C-terminal serine-rich region that contains several phosphorylation sites. Although some of these serines are shown to be phosphorylated in the resting state, further phosphorylation of specific residues is required for its activation and nuclear translocation. In uninfected cells, IRF-3 is present in the cytoplasm, however, following infection, it undergoes phosphorylation on serine 385, serine 386, threonine 390, and serine 396. These phosphorylation events induce a conformational change that leads to its dimerization and nuclear localization where it associates with CREB binding protein to form dsRNA activated factor 1 that subsequently leads to the activation of type I interferons and ISG genes. Phosphorylation and subsequent activation of IRF-3 shown to be inhibited by vaccinia virus protein E3. Mutations in IRF3 gene have been linked to infection-induced, Herpes-specific acute encephalopathy that is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Clone AR-1 recognizes an epitope within the DNA-binding domain of IRF-3. It can detect resting IRF-3 as well as the activated form. (Ref.: Rustagi, A., et al. (2013). Methods. 59(2); 225-232; Honda, K., et al. (2006). Immunity. 25(3); 349-360).

Especificidade

Clone AR1 is a mouse monoclonal antibody that detects Interferon regulatory factor 3 (IRF-3). It targets an epitope within the DNA-binding domain from the N-terminal half.

Imunogênio

His-tagged full-length human recombinant human Interferon regulatory factor 3 (IRF-3).

Aplicação

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-3 in A549 cell lysate.

Tested Applications

ELISA Analysis: A representative lot detected IRF-3 in ELISA applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Flow Cytometry Analysis: A representative lot detected IRF-3 in Flow Cytometry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Western Blotting Analysis: A representative lot detected IRF-3 in Western Blotting applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Immunocytochemistry Analysis: A representative lot detected IRF-3 in Immunocytochemistry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IRF-3, clone AR-1, Cat. No. MABF2778, is a mouse monoclonal antibody that detects Interferon regulatory factor 3 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Não está encontrando o produto certo?  

Experimente o nosso Ferramenta de seleção de produtos.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica