MABC570
Anti-Sec8 Antibody/Exoc4, clone 2E12
clone 2E12, from mouse
Sinônimo(s):
Exocyst complex component 4, Exocyst complex component Sec8, rSec8
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
2E12, monoclonal
reatividade de espécies
human, rat, mouse
técnica(s)
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG2bκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... EXOC4(60412)
Descrição geral
Sec8/Exoc4 is a component of the exocyst complex which is a multi-protein complex used for docking of exocytic vesicles with fusion sites on the plasma membrane. The exocyst complex is essential for exocytosis which is the process whereby proteins, hormones, transmitters etc. are transported and released to the extracellular environment. The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Sec8/Exoc4 appears to be important for tethering of secretory vesicles to specific regions on the plasma membrane. It seems particularly important in normal development for growth and morphological change by allowing the secretion of matrix metalloproteinases such as MMP2 or MMP9 in discrete locations. Knockdowns exhibit reduced cellular proliferation as well as invasive activities as well. As such Sec8/Exoc4 is important in tumor growth and invasion as well, thus it is also a current target for cancer therapy.
Imunogênio
Recombinant protein corresponding to rat Sec8/Exoc4.
Aplicação
Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sec8/Exoc4 in HeLa, A431, and NIH/3T3 cells. DyLight® is a registered trademark of Thermo Fisher Scientific.
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Sec8 Antibody/Exoc4, clone 2E12 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sec8/Exoc4.
Qualidade
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sec8/Exoc4 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sec8/Exoc4 in 10 µg of HeLa cell lysate.
Descrição-alvo
~110 kDa observed
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Outras notas
Concentration: Please refer to lot specific datasheet.
Informações legais
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Exo70 generates membrane curvature for morphogenesis and cell migration.
Zhao, Yuting, et al.
Developmental Cell, 26, 266-278 (2013)
Jianglan Liu et al.
Molecular biology of the cell, 18(11), 4483-4492 (2007-09-01)
The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma
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