MABC1150

Anti-MAGEA3 Antibody, clone 57B

clone 57B, from mouse

• Sinônimo(s): Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 57B, monoclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_005353
• nº de adesão UniProt: P43357
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MAGEA3(4102)

Descrição

Descrição geral

Melanoma-associated antigen 3 (UniProt: P43357; also known as Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen) is encoded by the MAGEA3 (also known as MAGE3) gene (Gene ID: 4102) in human. MAGE-3 is a member of the melanoma-associated antigen gene family of cancer antigens that are expressed in several neoplastic, but not normal, tissues with the exception of testes. In melanoma cells, it enhances viability and serves as an antigen for cytolytic T lymphocytes. MAGEA3 expression is not reported in kidney tumors, leukemias and lymphomas. MAGEA3 expression is normally limited to early developmental stages. It enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It is also reported to enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. It is proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Especificidade

Clone 57B specifically targets a sequence in the N-terminal region of melanoma associated antigen 3 in human testes.

Imunogênio

Epitope: domain of:

His-tagged recombinant human melanoma associated antigen 3 containing the polyhistidine tail.

Aplicação

Anti-MAGEA3 Antibody, clone 57B, Cat. No. MABC1150, detects multiple MAGE-A proteins. Validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected MAGEA3 in Immunocytochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Landry, C., et. al. (2000). Int J Cancer. 86(6):835-41; Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9).

Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).

Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.

Research Category
Apoptosis & Cancer

Qualidade

Evaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.

Descrição-alvo

34.75 kDa calculated.

forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1