MAB4603
Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03
clone 3CSP03, Chemicon®, from mouse
Sinônimo(s):
CPP32
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
3CSP03, monoclonal
reatividade de espécies
human
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2a
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... CASP3(836)
Especificidade
Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.
Imunogênio
Epitope: large subunit & proform
Recombinant full-length human caspase-3 protein
Aplicação
Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
Research Category
Apoptosis & Cancer
Metabolism
Apoptosis & Cancer
Metabolism
Research Sub Category
Caspases
Enzymes & Biochemistry
Caspases
Enzymes & Biochemistry
Western blot: 1 μg/ml for 2 hours at RT.
Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.
Optimal working dilutions must be determined by end user.
Ligação
Replaces: 04-440; 04-1088
forma física
Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Armazenamento e estabilidade
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
M Volm et al.
Anticancer research, 19(3A), 1669-1671 (1999-09-02)
In order to explore whether apoptosis is associated with angiogenesis in lung cancer, immunohistochemistry was employed to determine the pro-apoptotic factors Fas ligand (FasL) and caspase-3 (Cas-3) in 70 squamous cell lung carcinomas. Furthermore, the vascular endothelial growth factor (VEGF)
Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
Krajewska, M, et al.
Cancer Research, 57, 1605-1613 (1997)
A Srinivasan et al.
Cell death and differentiation, 5(12), 1004-1016 (1999-01-23)
Activation of caspase-3 requires proteolytic processing of the inactive zymogen into p18 and p12 subunits. We generated a rabbit polyclonal antiserum, CM1, which recognizes the p18 subunit of cleaved caspase-3 but not the zymogen. CM1 demonstrated an apparent specificity for
M Vakkala et al.
British journal of cancer, 81(4), 592-599 (1999-11-26)
The aim of this investigation was to study the expression of caspases 3, 6 and 8 and their association to apoptosis in preneoplastic and neoplastic lesions of the breast. The material consisted of nine benign breast epithelial hyperplasias, 15 atypical
Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma.
Virkajarvi, N, et al.
Histopathology, 33, 432-439 (1998)
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