MAB19101
Anti-Tenascin Antibody, clone DB7
clone DB7, Chemicon®, from mouse
Sinônimo(s):
Tenascin-C
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
DB7, monoclonal
reatividade de espécies
human, rabbit, guinea pig
não deve reagir com
rat, mouse
fabricante/nome comercial
Chemicon®
técnica(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG2a
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... TNC(3371)
Especificidade
The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein
Imunogênio
Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.
Aplicação
Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Ligação
Replaces: MAB1902
forma física
Format: Purified
The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
Armazenamento e estabilidade
Maintain refrigerated at 2-8°C
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia.
Kaarteenaho-Wiik, R, et al.
American Journal of Respiratory and Critical Care Medicine, 154, 511-518 (1996)
Tenascin expression in mucocutaneous diseases and related lesions of human oral mucosa.
Tiitta, O, et al.
Archives of Oral Biology, 40, 1039-1045 (1995)
Y Soini et al.
Journal of clinical pathology, 46(3), 218-221 (1993-03-01)
To determine the distribution of tenascin in normal and pathological bone marrow. 48 different bone marrow lesions were studied immunohistochemically using a monoclonal antibody to tenascin. Tenascin immunoreactivity was found in lesions with increased fibrosis and high numbers of reticular
Demonstration of tenascin-like immunoreactivity in rabbit corneal wounds.
Tervo, K, et al.
Acta Ophthalmologica, 67, 347-350 (1989)
Pleural mesotheliomas have an integrin profile distinct from visceral carcinomas.
Koukoulis, G K, et al.
Human Pathology, 28, 84-90 (1997)
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