HTS090RTA
Ready-to-Assay OT Oxytocin Receptor Frozen Cells
Human
Sinônimo(s):
Oxytocin-expressing cells
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About This Item
Produtos recomendados
Nome do produto
Ready-to-Assay OT Oxytocin Receptor Frozen Cells, Human OT GPCR frozen cells for Calcium Flux FLIPR Assays.
fonte biológica
human
Nível de qualidade
fabricante/nome comercial
Ready-to-Assay
técnica(s)
calcium flux assay: suitable
nº de adesão NCBI
método de detecção
fluorometric
Condições de expedição
dry ice
Descrição geral
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Oxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Millipore’s cloned human OT-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OT expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OT.
Oxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Millipore’s cloned human OT-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OT expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OT.
Descrição de linhagem celular
GPCR Cell Lines
Host cells: Chem-1
Ações bioquímicas/fisiológicas
GPCR Class: A
Protein Target: OT
Target Sub-Family: Oxytocin
Componentes
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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