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HGAMMAG-301K

Millipore

MILLIPLEX® Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.

Sinônimo(s):

Human Ig isotype multiplex panel, Human immune globulin multiplex panel, Human immunoglobulin multiplex kit, Luminex® human immunoglobulin immunoassay, Millipore Human immunoglobulin panel

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About This Item

Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Nível de qualidade

200

reatividade de espécies

human

fabricante/nome comercial

Milliplex®

assay range

accuracy: 88-99%
sensitivity: 0.08 ng/mL
(IgG4)
sensitivity: 0.09 ng/mL
(IgG3)
sensitivity: 0.47 ng/mL
(IgM)
sensitivity: 1.5 ng/mL
(IgA)
sensitivity: 10 ng/mL
(IgG1)
sensitivity: 85 ng/mL
(IgG2)
standard curve range: 0.2-150 ng/mL
(IgG3)
standard curve range: 0.4-300 ng/mL
(IgG4)
standard curve range: 14-10,000 ng/mL
(IgG1)
standard curve range: 2.1-1,500 ng/mL
(IgA)
standard curve range: 3.4-2,500 ng/mL
(IgM)
standard curve range: 41-30,000 ng/mL
(IgG2)

técnica(s)

multiplexing: suitable

método de detecção

fluorometric (Luminex xMAP)

Condições de expedição

wet ice

Categorias relacionadas

Descrição geral

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Five major isotypes have been identified in placental mammals: IgM, IgG, IgA, IgE and IgD (B-cell receptor) – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. In addition, isotyping applications include analyzing hybridomas during antibody generation and vaccine development.

The MILLIPLEX® Human Isotyping Bead Panel, measuring human IgG subclasses (1, 2, 3, 4), IgM and IgA is designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Aplicação

  • Analytes: IgG1, IgG2, IgG3, IgG4, IgA, IgM
  • Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples
  • Recommended Sample dilution: 1:16,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1μg/mL Ig per well
  • Assay Run Time: One day
  • Research Category: Inflammation & Immunology

Características e benefícios

Design your multiplex kit by choosing available analytes within this panel.

Componentes

MILLIPLEX MAP Anti-Human κ and λ Light Chain, PE

MILLIPLEX MAP Human Multi-Immunoglobulin Standard

MILLIPLEX MAP Human Immunoglobulin Positive Control

MILLIPLEX MAP Assay Buffer

MILLIPLEX MAP Wash Buffer, 10X

Informações legais

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Órgãos-alvo

Respiratory Tract

Código de classe de armazenamento

10 - Combustible liquids

Certificados de análise (COA)

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Nancy J Andersen et al.
Journal of immunological methods, 404, 24-32 (2013-12-18)
The study was designed to determine the sensitivity and reproducibility of recovering immunoglobulin (Ig) isotypes (IgG subclasses, IgA, IgE and IgM classes) from dried blood spots (DBS), a methodologic subcomponent of the Upstate KIDS Study. A multiplexed Luminex assay was
Brian K McFarlin et al.
Journal of dietary supplements, 10(3), 171-183 (2013-08-10)
Strenuous exercise, such as running a marathon, is known to suppress mucosal immunity for up to 24 hr, which can increase the risk of developing an upper respiratory tract infection (URTI) and reduced performance capacity (Allgrove JE, Geneen L, Latif
Sarah H Pedersen et al.
Clinical nutrition (Edinburgh, Scotland), 35(4), 871-879 (2015-06-18)
Understanding mature breastmilk immunology may benefit infants chronically exposed to infectious pathogens in resource-limited regions. This prospective rural/semi-rural Tanzanian cohort of women (n = 102 at delivery; 38% HIV-positive) and their infants (n = 102) investigated breastmilk, maternal and infant serum immunoglobulins (IgA/IgG1-4/IgM) and
Sophia Kwon et al.
Annals of the American Thoracic Society, 13(8), 1253-1261 (2016-04-21)
The World Trade Center (WTC) collapse generated caustic airborne particulates that caused chronic rhinosinusitis in exposed Fire Department of New York firefighters. Surgery was performed when symptoms remained uncontrolled despite medical management. To identify predictors of surgical intervention for chronic
Kristin L Boswell et al.
PLoS pathogens, 10(1), e1003853-e1003853 (2014-02-06)
The interaction between follicular T helper cells (TFH) and B cells in the lymph nodes and spleen has a major impact on the development of antigen-specific B cell responses during infection or vaccination. Recent studies described a functional equivalent of

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