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Key Documents

GR01L

Sigma-Aldrich

Anti-EGFR (Ab-1) Mouse mAb (528)

lyophilized, clone 528, Calbiochem®

Sinônimo(s):

Anti-Epidermal Growth Factor Receptor

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

528, monoclonal

forma

lyophilized

não contém

preservative

reatividade de espécies

human

não deve reagir com

rat, mouse

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

Isotipo

IgG2a

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Descrição geral

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Recognizes the EGF receptor protein in A431 cells and normal skin.

Imunogênio

partially purified, human EGFR from human A431 cells

Aplicação

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

Reconstituição

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Nota de análise

Positive Control
A-431 cells or skin tissue

Outras notas

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


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