GR01L
Anti-EGFR (Ab-1) Mouse mAb (528)
lyophilized, clone 528, Calbiochem®
Sinônimo(s):
Anti-Epidermal Growth Factor Receptor
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
528, monoclonal
forma
lyophilized
não contém
preservative
reatividade de espécies
human
não deve reagir com
rat, mouse
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Isotipo
IgG2a
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
Descrição geral
Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
Recognizes the EGF receptor protein in A431 cells and normal skin.
Imunogênio
partially purified, human EGFR from human A431 cells
Aplicação
Flow Cytometry (5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Advertência
Toxicity: Standard Handling (A)
forma física
Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
Reconstituição
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
Nota de análise
Positive Control
A-431 cells or skin tissue
A-431 cells or skin tissue
Outras notas
Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
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